The PLEX-ID BAC Detection assay is a qualitative, non-diagnostic assay intended for the detection and identification of Bacteria and Candida species, as well as four antibiotic resistance markers, through nucleic acid amplification and subsequent mass spectrometry analysis. The assay is designed to be used to identify individual organisms direct from sterile fluid or other matrices.
*Magnetic beads and clean-up reagents are included with the T5000 assay but must be purchased separately for use with the PLEX-ID assay.
The PLEX-ID BAC Detection assay kit includes:
- 10 Plates, 60 Assays
- Plate Processing Protocol
- DNA Dilution Buffer
A clean-up kit is included with the T5000 assay but must be purchased separately for use with the PLEX-ID assay.
Positive, negative, or extraction controls are not provided.
The PLEX-ID BAC Detection assay uses 13 primer pairs multiplexed into sixteen wells to detect and identify the following:
- All groups of bacteria including intracellular organisms like Mycoplasma, Chlamydia, and Rickettsia
- Candida species
- Antibiotic resistance markers:
- kpc – carbapenem resistance in Gram-negative bacteria
- mec A – methicillin resistance in Staphylococcus spp.
- van A – vancomycin resistance in Enterococcus spp.
- van B – vancomycin resistance in Enterococcus spp.
The assay is designed to be able to identify more than 3,400 species of bacteria, and more than 40 species of Candida, with reporting based upon the identification of a single species, a small cluster of indistinguishable species, or the closest known species in the database.
