The PLEX-ID MRSA Typing and Characterization assay is a qualitative, non-diagnostic assay intended for the detection of S. aureus from isolates through nucleic acid amplification and subsequent mass spectrometry analysis.
*Magnetic beads and clean-up reagents are included with the T5000 assay but must be purchased separately for use with the PLEX-ID assay.
The PLEX-ID MRSA assay kit includes:
- 10 Plates (120 Assays)
- DNA Dilution Buffer
- Plate Processing Protocol
- PCR Enzyme Dilution Buffer
A clean-up kit is included with the T5000 assay but must be purchased separately for use with the PLEX-ID assay.
Positive, negative, or extraction controls are not provided.
The PLEX-ID MRSA assay uses sixteen primer pairs multiplexed into eight wells to detect and identify the following:
- Staphylococci speciation
- Methicillin resistance
- Mupirocin resistance
- Panton-Valentine Leukocidin (PVL) virulence factor
- PVL type mutations associated with “R” and “H” genotypes of PVL
- Toxic Shock Syndrome Toxin 1
This assay is designed to identify and report more than 890 strains of Staph. aureus as individual species or as a cluster and MLST/genotype, PVL, resistance markers.
